The acid-fast stain is a laboratory test that determines if a sample of tissue, blood, or other body substance is infected with the bacteria that causes tuberculosis (TB) and other illnesses.
Q. What would happens if alcohol was used as the decolorizing agent in spore staining?
Spores have imperiate coat around them. It wash off the excess primary stain but not the spore. You used acid-alcohol as the decolorizing agent. It will removes stain, bacteria, and spores.
Table of Contents
- Q. What would happens if alcohol was used as the decolorizing agent in spore staining?
- Q. Is alcohol a decolorizing agent?
- Q. What if you used safranin as the primary stain and malachite green as the Counterstain?
- Q. Which decolorizing agent would you use for the acid fast staining procedure?
- Q. What diseases are diagnosed by using the acid fast stain?
- Q. What type of bacteria are capable of being an Endospore former or acid fast?
Q. Is alcohol a decolorizing agent?
Decolorizer-Alcohol Either acetone or ethyl alcohol can be used as the decolorizing agent. The alcohol dissolves lipids found in the outer cell membrane of Gram-negative bacteria, allowing the crystal violet-iodine complex to leak out of the thinner peptidoglycan layer.
Q. What if you used safranin as the primary stain and malachite green as the Counterstain?
If you use safranin as the primary stain and malachite green as the counterstain, then the cells will stain green and the spore will stain red.
Q. Which decolorizing agent would you use for the acid fast staining procedure?
Principle of Acid-Fast Stain Then the smear is decolorized with decolorizing agent (3% HCL in 95% alcohol) but the acid fast cells are resistant due to the presence of large amount of lipoidal material in their cell wall which prevents the penetration of decolorizing solution.
Q. What diseases are diagnosed by using the acid fast stain?
No test preparation is needed, except to rinse the mouth with water before collecting the sputum sample. AFB testing may be used to detect several different types of acid-fast bacilli, but it is most commonly used to identify an active tuberculosis (TB) infection.
Q. What type of bacteria are capable of being an Endospore former or acid fast?
Most types of bacteria cannot change to the endospore form. Examples of bacterial species that can form endospores include Bacillus cereus, Bacillus anthracis, Bacillus thuringiensis, Clostridium botulinum, and Clostridium tetani.
