More than 60 of the world’s leading science academies have called for a UN ban on the reproductive cloning of humans, to prevent the exploitation of vulnerable people.

List of Therapeutic Cloning Pros

• It has the potential to create organs.
• Tissue rejection is no longer a threat.
• It may help to treat genetic diseases.
• Donor items would no longer be necessary.
• It could lead to organ regeneration.
• It can act as a preventative treatment.

The main ethical roadblock against therapeutic cloning is the destruction of the generated embryos in order to collect cells that would further be differentiated in vitro.

Organism cloning. Organism cloning (also called reproductive cloning) refers to the procedure of creating a new multicellular organism, genetically identical to another. In essence this form of cloning is an asexual method of reproduction, where fertilization or inter-gamete contact does not take place.

There are two kinds of λ phage vectors – insertion vector and replacement vector. Insertion vectors contain a unique cleavage site whereby foreign DNA with size of 5–11 kb may be inserted.

Types of Vectors List

• Zero Vector.
• Unit Vector.
• Position Vector.
• Co-initial Vector.
• Like and Unlike Vectors.
• Co-planar Vector.
• Collinear Vector.
• Equal Vector.

ampicillin resistance

Yeast artificial chromosome (YAC) cloning systems enable the cloning of DNA stretches of 50 to well over 2000 kb. This makes it possible to study large intact regions of DNA in detail, by restriction mapping the YAC to produce a physical map and by examining the YAC for coding sequences or genes.