Plaques are clear zones formed in a lawn of cells due to lysis by phage. The morphology of the plaque depends upon the phage, the host, and the growth conditions. Usually phage infection is studied in a layer of soft agar (or “top agar”) which allows the phage to diffuse rapidly.
Q. How are bacteriophages counted?
Traditionally, three methods have been used to quantitate bacteriophages: (1) plaque counts on agar plates seeded with the bacteria in which the phages can propagate, (2) a dilution method, where bacterial lysis is used as an indicator of phage presence and (3) measuring the length of time required to lyse a …
Table of Contents
- Q. How are bacteriophages counted?
- Q. Why is soft agar used in plaque assay?
- Q. What is the meaning of bacteriophage?
- Q. How do you use bacteriophage in a sentence?
- Q. How can bacteriophage be used in medicine?
- Q. How do you use capsid in a sentence?
- Q. How do you spell bacteriophage?
- Q. What is the definition of a plasmid?
- Q. What is virulent phage?
- Q. How does a virus reproduce?
Q. Why is soft agar used in plaque assay?
Plaques do not continue to spread indefinitely. The medium used in phage plaque assays has a relatively low percentage of agar and therefore is called soft agar; it permits diffusion of phage to nearby uninfected cells but does not permit new phages to move to remote parts of the plate.
Q. What is the meaning of bacteriophage?
A bacteriophage is a type of virus that infects bacteria. In fact, the word “bacteriophage” literally means “bacteria eater,” because bacteriophages destroy their host cells. All bacteriophages are composed of a nucleic acid molecule that is surrounded by a protein structure.
Q. How do you use bacteriophage in a sentence?
Bacteriophage sentence example
- Delbrueck chose to use bacteriophage , viruses that infect bacteria and then can multiply very rapidly.
- bacteriophage lambda.
- bacteriophage genome) is not modified & is thus digested by the restriction enzymes & destroyed.
- bacteriophage virus.
- bacteriophage lambda assembly.
Q. How can bacteriophage be used in medicine?
Phage therapy is the use of bacteriophages to treat bacterial infections. This could be used as an alternative to antibiotics when bacteria develop resistance. Superbugs that are immune to multiple types of drugs are becoming a concern with the more frequent use of antibiotics.
Q. How do you use capsid in a sentence?
Capsid sentence example The hybridization data are consistent with partial sequence analysis of the AC1 gene and the capsid protein gene. capsid antigens in adenoviruses and AAV. Pests The only pest on lavender is the green capsid bug or cuckoo spit.
Q. How do you spell bacteriophage?
noun. any of a group of viruses that infect specific bacteria, usually causing their disintegration or dissolution.
Q. What is the definition of a plasmid?
A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell’s chromosomal DNA. Plasmids naturally exist in bacterial cells, and they also occur in some eukaryotes. Often, the genes carried in plasmids provide bacteria with genetic advantages, such as antibiotic resistance.
Q. What is virulent phage?
virulent phage A bacteriophage that causes the destruction of the host bacterium by lysis. Temperate phages, on the other hand, rarely cause lysis. A Dictionary of Plant Sciences.
Q. How does a virus reproduce?
A virus is a tiny, infectious particle that can reproduce only by infecting a host cell. Viruses “commandeer” the host cell and use its resources to make more viruses, basically reprogramming it to become a virus factory. Because they can’t reproduce by themselves (without a host), viruses are not considered living.