37 degrees C.

Effect of Temperature: Enzymes are most sensitive to heat in dilute solution and in the absence of substrate. The conversion of starch by a-Amylase increases in rate with rising temperature to a maximum of about 80oC. Heating above this temperature begins to destroy the amylase.

Below a certain temperature (149°F), alpha-amylase activity is low and so the large starch molecules remain insoluble because the enzyme is unable to break them up.

The various factors affecting amylase production was assayed which include pH, different substrate, temperature, natural source and additives. Results showed that pH 7.0 and 37ºC are found to be optimum values for both the growth of the isolate and max.

pancreas

From the 1 minute experiments we concluded that amylase works better at extreme hot temperatures rather than extreme cold temperatures and it works best around body temperature but the enzyme takes about 1 minute to break down all starch.

Add the amylase to the starch in the 0°C water bath. Every minute remove a sample of the starch-amylase solution and add it to a drop of iodine on the spotting tile. Repeat step 8 until the iodine no longer changes colour – meaning that there is no starch present, in other words the amylase has broken all starch down.

The Role of the Amylase Enzyme The end goal of amylase is to break down carbohydrates into simple sugars that the body can use for energy, and this starts in the mouth. As food is chewed and mixed with saliva, amylase starts working to break down food into smaller molecules (1).

Amylases: Break down starch into sugars like glucose and maltose.

Enzymes can break down nutrients into small, soluble molecules that can be absorbed. For example, amylase causes the breakdown of starch into simple sugars….Where enzymes are produced.

If your results show an abnormal level of amylase in your blood or urine, it may mean you have a disorder of the pancreas or other medical condition. High levels of amylase may indicate: Acute pancreatitis, a sudden and severe inflammation of the pancreas.

Avoid alcohol. Alcohol use will irritate your pancreas and liver, and may cause interactions with medications. Follow a diet that is low in fat, low in red meat, and high in fiber.

Timing. In acute pancreatitis, amylase can rise rapidly within 3–6 hours of the onset of symptoms, and may remain elevated for up to five days. However, it has a short half-life of 12 hours so the concentration can normalise within 24 hours.

Acute pancreatitis is an inflammation of the pancreas. It is painful, develops quickly, and it can, in some cases, be fatal. Some mild cases resolve without treatment, but severe, acute pancreatitis can trigger potentially fatal complications.