Quantitative reverse transcription PCR (RT-qPCR) is used when the starting material is RNA. In this method, RNA is first transcribed into complementary DNA (cDNA) by reverse transcriptase from total RNA or messenger RNA (mRNA). The cDNA is then used as the template for the qPCR reaction.
Q. What is the PCR method used for?
Polymerase chain reaction (PCR) is a technique used to “amplify” small segments of DNA.
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Q. How do you explain PCR?
Polymerase chain reaction, or PCR, is a technique to make many copies of a specific DNA region in vitro (in a test tube rather than an organism). PCR relies on a thermostable DNA polymerase, Taq polymerase, and requires DNA primers designed specifically for the DNA region of interest.
Q. What is the basic principle of PCR?
Polymerase chain reaction (PCR) is a technology used for quick and easy amplifying DNA sequences, which is based on the principle of enzymatic replication of the nucleic acids. This method has in the field of molecular biology an irreplaceable role and constitutes one of the basic methods for DNA analysis.
Q. Why is a PCR test better?
PCR testing is considered the “gold standard” in SARS-CoV-2 detection. This test actually detects RNA (or genetic material) that is specific to the virus and can detect the virus within days of infection, even those who have no symptoms.
Q. What is real-time PCR principle?
The principle of real-time PCR relies on the use of fluorescent dye. In general, the principle of the present method is stated below, “The amount of the nucleic acid present into the sample is quantified using the fluorescent dye or using the fluorescent labeled oligos.” DNA binding dye (Intercalating dye-based method)
Q. What is the advantage of real time PCR?
One of the primary advantages of real-time PCR is the ability to identify amplified fragments during the PCR process. Real-time PCR measures the amount of the product during the exponential phase whereas standard PCR measures product during the plateau phase.
