As nouns the difference between spectrophotometer and spectrofluorometer. is that spectrophotometer is (physics) an instrument used to measure the intensity of electromagnetic radiation at different wavelengths while spectrofluorometer is (physics|chemistry) a spectrometer used in fluorescence spectroscopy.

fluorometer
Fluorescence is measurable by fluorometers. A fluorometer is an instrument designed to measure the various parameters of fluorescence, including its intensity and wavelength distribution of the emission after excitation. Chemists use this to identify properties and the amount of specific molecules in a sample.

Among the most common light source for fluorometers is the low-pressure mercury lamp. This provides many excitation wavelengths, making it the most versatile. However, this lamp is not a continuous source of radiation. The xenon arc lamp is used when a continuous source of radiation is needed.

Spectrofluorometers (or fluorescence spectrophotometers) measure the fluorescence signature of an analyte in a sample based on its specific excitation and emission wavelengths. The fluorescence signature can be correlated to the concentration level of the analyte in the sample.

The essential components of a spectrofluorometer are a light source, an excitation monochromator, a sample cell/cuvette, an emission monochromator and a detector.

Fluorometer. These parameters are used to identify the presence and the amount of specific molecules in a medium. Modern fluorometers are capable of detecting fluorescent molecule concentrations as low as 1 part per trillion.

Aquaculture operations such as fish farms us fluorometers to measure food availability for filter feeding animals like mussels and to detect the onset of Harmful Algal Blooms (HABs) and/or ” red tides ” (not necessarily the same thing). Fluorometers can be used to determine the nucleic acid concentration in a sample.

Two general types of instruments exist: filter fluorometers that use filters to isolate the incident light and fluorescent light and spectrofluorometers that use a diffraction grating monochromators to isolate the incident light and fluorescent light.

Not to be confused with X-ray fluorescence. A fluorometer or fluorimeter is a device used to measure parameters of visible spectrum fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light.