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Tryptic Soy Broth (TSB) is a nutritious medium that will support the growth of a wide variety of microorganisms, especially common aerobic and facultatively anaerobic bacteria.

To prepare TSB, the following ingredients are dissolved under gentle heat and then autoclaved for 15 minutes at 121 °C (250 °F).

1. 17 grams (0.60 oz) of Tryptone.
2. 3 grams (0.11 oz) of Soy.
3. 5 grams (0.18 oz) of NaCl.
4. 2.5 grams (0.088 oz) of dipotassium phosphate (K2HPO4)
5. 2.5 grams (0.088 oz) of glucose.

Trypticase soy agar or tryptone soya agar (TSA) and Trypticase soy broth or tryptone soya broth (TSB) with agar are growth media for the culturing of bacteria. They are general-purpose, nonselective media providing enough nutrients to allow for a wide variety of microorganisms to grow.

MilliporeSigma is an American chemical, life science and biotechnology company, before 2014 known as Sigma-Aldrich, owned by Merck KGaA. The company is currently a part of Merck’s life science business and in combination with Merck’s earlier acquired Millipore, operates as MilliporeSigma. …

The acid phosphate (HPO42-), buffering agent present in the TSB, is part of the polyfunctional system of phosphoric acid whose pKas (at 25 ° C) are 2.148; 7.199 and 12.15. Buffering could be obtained in the approximate ranges 3.8-5.8 and 5.4-7.4.

Tryptic Soy Broth (TSB), commonly referred to as soybean casein digest, is a general purpose medium rather than a selective or differential/indicator medium.

TSB Medium Preparation

1. Weight 3 gm of Tryptic soy broth (TSB) powder and dissolve in 100 ml distilled water.
2. Place the bottle on magnetic stirrer to mix.
3. Aliquot 10 ml of the medium to each 13*100 mm glass spiral tubes (or other suitable containers as per your need).

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